DOT Drug Test - info-hero

DOT Drug Test

Find Clinic Now

Whether you're hiring for trucking, shipping, aviation, rail, transit, or merchant marine roles, DOT drug testing is required for safety-sensitive employees—and it's not something you want to get wrong. From pre-employment DOT drug tests to random drug testing pools, Health Street makes it easy to stay in compliance with DOT regulations.

With fast scheduling, thousands of DOT drug testing sites near you, and support for urine DOT drug tests, we serve both employers and individuals who need to meet federal transportation drug testing requirements.

Wondering how much a DOT drug test costs, where to get a DOT drug test, or how long a DOT drug test takes? This page covers everything you need to know. Register online or call (888) 378-2499 to schedule a DOT drug screen near you.

Workforce Screening, Digitally Delivered

Fast Efficient Service

Friendly staff. Fast efficient service
Yosawn G.
6/13/2025
EmCo Transportation

Professional, Friendly

They are very professional. Friendly. Great people
Marvin G.
6/13/2025
MJ TEAM

Great Service

Great customer service.
Brenda W.
6/13/2025
Constable Sanitation LLC

Very Kind

She was very kind
Hamza S.
6/13/2025
Al-Maauun

Totally Satisfied, Professional

Muy amables todos, totalmente satisfecho con el trato profesional de los que me atendieron
ARNALDO A.
6/12/2025
Arsh Brothers Inc
Star Rating
4.9 out of 5 stars (967 reviews)

Find a Health Street Clinic

Frequently Asked Questions

How long does it take for a DOT drug test to come back?

The DOT pre-employment drug test time frame is fast. Negative urine drug tests typically come back the next business day, but in some cases can take longer. If the drug screening is not negative, further testing is required. Our lab will run a GC/MS confirmation test on all non-negatives, which provides an official, quantitative measurement of the level of drugs in the system. Confirmation testing takes approximately 3-5 days.

Why is my DOT drug test taking so long?

Most negative DOT drug test results are returned within one business day. However, delays can happen for a few reasons. If the test is non-negative, it must go through a more detailed GC/MS confirmation process, which can take an additional 3–5 days. Delays can also occur due to issues like incomplete paperwork, specimen temperature concerns, or if the Medical Review Officer (MRO) needs to contact the employee for more information, such as verifying prescriptions.

Who needs a DOT drug screen?

All transportation industry modalities require DOT drug screening for various purposes. For example, trucking companies must ensure safety-sensitive staff, such as drivers, are tested on a pre-employment and random basis, as well as for post-accident, reasonable suspicion, return-to-duty, and follow-up testing when indicated.

Who pays for the DOT drug test?

DOT regulations are silent about who is responsible for paying for drug testing. Typically, the drug testing for trucking companies is covered by the company, but not always. However, if a driver fails a test, smaller companies are much less likely to pay for a driver's return-to-duty and follow-up testing, putting the onus on the driver to pay for the tests.

Large fleets, on the other hand, often pay for all logistics drug testing, including return-to-duty and follow-up testing, especially if the drivers are unionized.

Is return-to-duty testing required?

Return to duty tests are only required if a person has failed a test, sought out the counsel of a Substance Abuse Professional, and was subsequently cleared to return to duty by the SAP. DOT regulations require trucking firms to ensure that drivers who are subject to the return-to-duty and follow up testing are complying with those testing protocols, including passing all tests as outlined by the Substance Abuse Professional.

Who reviews the DOT drug test results?

DOT drug tests are reviewed by Medical Review Officers (MRO). An MRO signature is required on all valid DOT drug tests, whether positive or negative.

What information does Health Street need so I can send the driver for the test? To schedule a drug test with Health Street, we will ask for the following information during the registration process:

arrow_right
Company name
arrow_right
Employee name
arrow_right
ZIP code
What happens if you fail a DOT physical drug test?

If you fail a DOT drug test, please review the information provided by the FMCSA (Federal Motor Carrier Safety Administration) to learn more about the next steps. Health Street is available to assist you in scheduling DOT drug tests as required.

What is a DOT Physical?

A DOT physical is a medical exam required for certain workers, like truck drivers, to ensure they meet health and safety standards. It includes vision and hearing tests, a blood test, and a urinalysis. Some packages also combine the DOT physical with a DOT drug test.

About Our DOT Drug Test

Health Street offers DOT-compliant trucking drug testing for drivers, pilots, rail workers, maritime employees, and others in transportation and logistics. We provide easy access to DOT drug testing facilities near you, so you can schedule a urine DOT drug test with just a few clicks.

Reasons for Drug Testing

DOT drug and alcohol tests are administered under the federal authority of the agency overseeing the industry in which a company operates. DOT and its agencies define the reasons that prompt the need for tests. The opposite is also true. DOT drug tests cannot be administered unless they fall into one of the predefined categories. The following are reasons when DOT drug tests must be given:

  • Pre-employment DOT drug tests
  • Random testing through a DOT consortium or company pool
  • Post-accident drug screens
  • Reasonable suspicion testing
  • Return-to-duty and follow-up DOT testing

Reviewing Drug Test Results

The result of a DOT drug test is reported in a secure PDF file which details the list of the tested drugs. For each class of drugs, there is an indication of positive or negative. If a drug comes back positive, the specific metabolite which tested positive will be indicated. The PDF file can be easily downloaded from our portal for printing or forwarding.

Why Choose Health Street

Not all DOT drug testing companies put your needs first, but we do. At Health Street, we offer:

  • Safe, secure, and fast drug testing with accurate results from licensed labs
  • Nationwide network of more than 10,000 DOT drug test locations
  • Mobile drug testing available for companies and individuals
  • Secure portal for easy test management and viewing of results
  • Trusted provider in the drug testing industry

What Drugs Does the Urine DOT Drug Test Cover?

The following substances can be detected in our DOT Drug Test. Use the below links for more information about each substance:

Related Tests

If you'd like to see a comprehensive list of all DOT tests that Health Street offers, you can view all DOT tests and services here.

If you're looking for something else, try taking a look at our DOT Random Pool Drug Testing.

DOT Compliance Resources

Below are some resources to learn more about compliance for the Department of Transportation (DOT), Federal Motor Carrier Safety Administration (FMCSA), United States Coast Guard (USCG), Federal Aviation Administration (FAA), and Pipeline and Hazardous Materials Safety Administration (PHMSA).

Reviews

No Problems

Star Rating
By Edward H. on 6/12/2025
MJ TEAM
There were no problems just time consuming

Professional & Respectful

Star Rating
By DERRICK S. on 6/11/2025
Rested Care Management
Very professional kind an respectful

Fast, Easy

Star Rating
By Amanda W. on 6/11/2025
Hi Pro Inc
Fast and easy

Do Awesome

Star Rating
By JUSTIN M. on 6/11/2025
DT EXPRESS LLC
Do awesome

Quick, Efficient

Star Rating
By Dylan E. on 6/11/2025
Spirit Logistics Network Inc
Very Quick and efficient. Nice employee's working there.

Nice, Polite Workers

Star Rating
By AMIRKHON D. on 6/11/2025
UZFER EXPRESS LLC
Very nice and polite workers

Friendly, Professional Staff

Star Rating
By Travis P. on 6/11/2025
Custom Fleet Maintenance Inc
The staff was friendly and professional. The wait time was short, in and out in less than a half hour

Awesome Job

Star Rating
By Roytheric H. on 6/11/2025
JL Barnett Trucking
Walk in Monday for a dot urine test,within 30min finishing and two days later my results came back ..awesome job

Quick, Easy Process

Star Rating
By Shakenya M. on 6/11/2025
NFA Environmental
It was quick and easy process

Great, Nice

Star Rating
By LUCKA P. on 6/10/2025
FRANCHISE - TOMMY
Great . Tia was very nice