Person using a breathalyzer device outdoors with clear sky in the background.

DOT Alcohol Test

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Health Street provides DOT breath alcohol testing compliant with 49 CFR Part 40 for CDL drivers, pilots, ship captains, and other safety-sensitive employees. Our DOT alcohol tests are performed by certified screening test technicians using federally approved evidential breath testing (breathalyzer) devices to ensure public safety and regulatory compliance.

Testing is available at over 14,000 clinics nationwide. Scheduling is fast and easy. Register online or call (888) 378-2499. After registration, the person being tested will instantly receive an authorization barcode and clinic map via email or text. They should simply present the barcode and a photo ID at the selected alcohol testing lab. Mobile post-accident testing is also available in most areas. Results are delivered fast, signed by a doctor securely reported online.

Workforce Screening, Digitally Delivered

“ Great Job ”

Great job
Danny M.
9/16/2026
TN SITE LLC

“ Excellent Professional Staff ”

Excellent staff very friendly, polite & professional
Victor P.
9/4/2026
HL Motor Group

“ Good Quick Fast ”

Good quick and fast
Surinderpal S.
8/28/2026
R Millennium Transport Inc

“ Fast, Friendly Service ”

Very clean and organized, very fast service, and best of all, the staff are extremely friendly!
Giorgi J.
8/28/2026
Millenium Express LLC

“ Very Professional ”

Very professional
Scott G.
8/17/2026
982430 Alberta Ltd
Star Rating
4.9 out of 5 stars (94 reviews)

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DOT Breath Alcohol Testing Options

DOT Breath Alcohol Test

$99
For all employees subject to DOT drug and alcohol regulations. Uses an approved EBT to accurately measure alcohol in breath. Find Clinic Now

Package: DOT Drug Test + DOT Breath Alcohol

$190
Includes both alcohol breath test and DOT drug test for complete compliance. Find Clinic Now

Frequently Asked Questions

Below are the most commonly asked questions about this product in our call center.

What is DOT breath alcohol testing?

A DOT alcohol test is a breath alcohol test required by the Department of Transportation for safety-sensitive employees. The process follows 49 CFR Part 40 and uses approved devices and certified technicians for accurate results.

Does Health Street provide mobile services for post-accident drug and alcohol testing?

Yes, mobile post-accident drug and alcohol testing is available in many locations. This service lets you test employees at the site of an incident or accident.

Is alcohol screening part of DOT testing?

DOT regulations state that there are specific cases where alcohol testing is required, and that a candidate or employee may be tested for alcohol use using breath or saliva tests. Urine samples are used for drug testing.

What is the cost of an alcohol breath test?

Health Street provides DOT alcohol testing beginning at $99. If a DOT drug test is added, the cost is $190 .

How do I order an alcohol test for DOT?

Register online or call us. After registration, the person being tested gets a barcode and clinic address. They present their barcode and ID to the technician, who performs the test.

How fast are DOT alcohol test results available?

Negative results are available instantly. The technician will provide the result right after the test is complete. If the result is non-negative, a confirmation test will be performed after a fifteen-minute waiting period.

Who needs to take a DOT alcohol test?

The DOT alcohol screens are specifically designed for individuals in safety-sensitive positions, such as CDL drivers (commercial drivers), pilots, ship captains, and other employees whose roles directly impact public safety. This test is used in specific circumstances to ensure compliance with regulations and maintain public safety.

Can I schedule a DOT alcohol test for someone else?

Yes. Whether you're a company or a family member, you can preregister someone else. You'll just need their name and a method of payment.

Can employers order DOT alcohol tests for employees?

Yes. Employers can set up a company account, register employee tests, and view results through our secure GLASS portal. You can also enroll your drivers in our DOT random testing consortium for annual compliance.

About Our DOT Alcohol Test

What is a DOT Breath Alcohol Test?

A DOT breath alcohol test (also called a DOT alcohol test) is an alcohol breath test required by the Department of Transportation for employees in safety-sensitive positions.

Testing procedures are outlined in 49 CFR Part 40 and require the use of a breath or saliva device approved by the National Highway Traffic Safety Administration (NHTSA). Initial screenings and confirmation tests must be performed by a qualified breath alcohol technician (BAT) or screening test technician (STT).

Reasons for DOT Drug and Alcohol Testing

Department of Transportation drug and alcohol tests are administered under the federal authority of the agency that oversees the company's industry. The department and its agencies define the reasons for drug and alcohol tests.

A DOT drug test cannot be legally administered unless it falls into one of these categories:

  • Pre-employment
  • Random testing (as part of an alcohol testing program)
  • Post-Accident
  • Reasonable Suspicion
  • Return to duty
  • Follow up

Evidential Breath Testing (EBTs)

At Health Street, our DOT mandated alcohol testing is performed using federally approved Evidential Breath Testers (EBTs). These breath alcohol devices must only register alcohol use in a person's breath, and not any other volatile substance like acetone, for Department of Transportation approval. Approved testing devices are listed on the federal register.

Results of an approved and properly administered DOT breath alcohol screening test will never yield a false positive for alcohol consumption. This means it can be used as legal evidence in court.

About Breath Alcohol Test Results

For negative screenings, Breath Alcohol Tests give you instant results. Technicians use a DOT Alcohol Testing Form (ATF) to record test results. If the screening is non-negative, the person will wait a period of 15 minutes. After the wait period, there will be a confirmation test—again, with instant results. In these cases, only the confirmation test results count.

Test results over 0.020 BAC (Blood Alcohol Concentration) are considered positive, indicating that the employee must be immediately removed from safety-sensitive duties because of the amount of alcohol in their system. A result exceeding 0.040 is even more serious. The employee must be approved to return to work by a substance abuse professional.

Related Tests

Need a different testing option?

If you'd like to see a comprehensive list of all DOT tests that Health Street offers, you can view all DOT tests and services here.

If the DOT Alcohol Test is not what you need, consider trying one of the following options:

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Recommend Health Street

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I would recommend Health Street

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CE&A Professional Services
Thanks for the quick work!

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Ryan did really good. He answer all my questions. Thanks for the service

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Check Drugs Inc
Had conversation in a good and polite way. Thank you Ryan and Health-Street team.

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By Bill Chris H. on 6/20/2025
C & V Holmes Trucking LLC
Louis was very professional and helped educate me. It is rare to be able to call a company and actually speak to someone immediately. He was courteous and has such a wonderful personality for customer service. Thank you Louis!!

Super Easy Change

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By James B. on 6/12/2025
Innovative Building Concepts Inc
He made the location change for a DOT Alcohol test super easy!

Quick, Helpful

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Hallas Landscapes
Quick and helpful

Excellent Service

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Walbert Trucking
Excellent service

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