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DOT Drug Test

A DOT Drug Test is for truckers, pilots, captains, and safety-sensitive employees. Pre-employment, Random, & Post-Accident testing is available. Employers and individuals can find a location for a DOT drug test and register online, or call (888) 378-2499 to set up testing for staff or others. We offer DOT drug testing services at over 5000 clinic locations nationwide.

Our online registration process is simple. You choose the lab location based on ZIP code during registration. An authorization barcode is instantly emailed to you and texted directly to the phone of the person being tested. A map of the clinic location will accompany the barcode. The registrant can then walk in to the drug testing facility and show the barcode along with photo ID. Results are fast, signed by a doctor, and reported securely online.

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4.9 out of 5 stars (387 reviews)
$79 for Random Pool Members
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Fantastic service! Very fast, efficient, and easy. They made finding locations for my employees a snap, and Kept me informed of every status change in the process. Our company will DEFINITELY be using Health Street again.
By Rick Woosley on August 18, 2017
Health Street was SO HELPFUL - I needed immediate assistance since I had a candidate waiting at a facility for a DOT test and needed to set up and make a payment asap. Because of their assistance, she did not need to reschedule her appointment. Very much appreciated!
By Nicole on May 16, 2016
Logo of Health Street's client, Barnes Aerospace
Easy, efficient and the staff was very helpful and great to talk with. Very approachable. This was a very smooth move and we are excited that we have you as a resource. Super easy to maneuver. Straight forward, very refreshing. No issues. Every aspect of this was convenient and very quick and easy. Good people, good software, no stress and quick results. Win, Win!
By Kerry on November 14, 2019
Outwest Express
Outwest Express's Logo
4.9 out of 5 stars (385 reviews)

About DOT Drug Test

List of drugs detected by DOT Drug Test

Reasons for Drug Testing

DOT Drug and alcohol tests are administered under the federal authority of the agency overseeing the industry in which a company operates. DOT and its agencies define the reasons which prompt the need for tests. The opposite is also true. DOT drug tests cannot be administered unless it falls into one of the pre-defined categories. The following are reasons when DOT drug tests must be given:
  • Pre-employment
  • Random
  • Post Accident
  • Reasonable Suspicion
  • Return to duty
  • Follow up

SAMHSA Certified Laboratory and MRO

Like all of our lab based tests, DOT Drug Tests are processed at a SAMHSA Certified laboratory. Results are reviewed by one of our Medical Review Officers (MRO). Our MROs are licensed physicians and toxicology experts. If the result of a urine drug test is non-negative, the MRO will reach out to the person to ask about prescription medications and, if appropriate, to request proof. Once that conversation with the doctor takes place, and the person has a chance to provide proof of any prescriptions, the MRO will release the final results, which will be reported to the Employer or person who requested the test.

Reviewing Drug Test Results

The result of a DOT Drug Test is reported in a secure PDF files which detail the list of the tested drugs. For each class of drugs, there is an indication of Positive or Negative. If a drug comes back positive, the specific metabolite which tested positive will be specifically indicated. Quantitative levels for positive results are available upon request. The PDF file can be easily downloaded from our portal, printed, or forwarded. We are also happy to send or fax drug test results to any person, employer, or organization that needs to get a copy of the report.

How long do drug test results take?

Negative urine drug tests typically come back the next business day, but in some cases can take longer. If the drug screening is not negative, further testing is required. Our lab will run a GC/MS confirmation test on all non-negatives, which provides an official, quantitative measurement of the level of drugs in the system. Confirmation testing takes approximately 3-5 days.

Staff Glass™... the Employer eResults Portal

Business owners get free access to our secure Employer eResults Portal, StaffGlass, to place drug test orders, view test results, manage employee screenings and eSignature documents (such as policies and procedures), update random drug testing pools, and register for background checks. Non-business clients get results via email and/or fax.

How it Works

  • Registration booklet with penRegister Online or By Phone
  • Right pointing arrow
  • Patient seated next to clinic practitionerVisit a Health Street Clinic
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  • Magnifying glass over computer screen inspecting checkmarkGet Your Fast, Accurate Results


387 Ratings
4.9 out of 5 stars.
DOT Drug Test
By Rubén Leyva on September 26, 2020
Good service
DOT Drug Test
By David Vanvekoven on September 19, 2020
Customer service was excellent Results were very rapid thank you
DOT Drug Test
By Neal Wright on September 16, 2020
Carolina Freight Ways
Very good communication, Best I've seen.
DOT Drug Test
By Candace on September 8, 2020
KS Fabricating Group
Excellent customer service and a quick turn around time. Health Street made it such an easy process.
DOT Drug Test
By Walter Kitsmillet on September 3, 2020
Efficient and professional. Awesome Place.
DOT Drug Test
By Gary MvGregor on August 20, 2020
Very quick results and efficient!
DOT Drug Test
By Steve Rakkar on August 20, 2020
The service was excellent. I thought it was a little pricey, for the drug screen. But they were extremely efficient. They had my drug screen organized within minutes, with an identifying bar code. I walked it and got it done. Within 56hrs. results were ready, and a specialized form for the Coast Guard was completed, signed, and emailed to me. Impressive.
DOT Drug Test
By ED Jannicelli on August 13, 2020
fast and easy
DOT Drug Test
By Brenda Fulton on August 12, 2020
Hockley Transport LLC
Quick and simple.
DOT Drug Test
By Julie Randall on August 10, 2020
Sacred Wind Communications
Customer Service is always awesome and A-1. The staff is pleasant, helpful and professional. We've been going to HealthStreet for over a year and it's a 1 stop place for anything you need.Thank you to the staff!!!!

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