DOT Drug Test - info-hero

DOT Drug Test

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A DOT Drug Test is for truckers, pilots, captains, and safety-sensitive employees governed by DOT regulations in all transportation modalities. Pre-employment, Random, Reasonable Suspicon & Post-Accident testing are available, as well as Return to Duty and Follow-Up testing. Employers and individuals can find a location for a DOT drug test and register online, or call (888) 378-2499 to set up testing for staff or others. We offer DOT drug testing services at over 10,000 clinic locations nationwide.

Our online registration process is simple. You choose the lab location based on ZIP code during registration. An authorization barcode is instantly emailed to you and texted directly to the phone of the person being tested. A map of the clinic location will accompany the barcode. The registrant can then walk in to the drug testing facility and show the barcode along with photo ID. Results are fast, signed by a doctor, and reported securely online.

Workforce Screening, Digitally Delivered

Fast, Efficient
Fast and efficient
Tyler P.
McDonagh Demolition Inc - DOT
Hassle Free
Hassle free
Shadan A.
Xat Logistics Llc
Fast Service
Cindy J.
Partners 5 Hauling Inc
Rapid Results
Shannon R.
Speedy Results
Very speedy results.
Harold .
Star Rating
4.9 out of 5 stars (445 reviews)

DOT Drug Test Options

DOT Drug Test
Health Street's DOT Drug Test complies with DOT regulations for drug testing of safety-sensitive workers in transportation industries, such as trucking, shipping, aviation, piplelines and hazardous materials. Register Now
DOT Drug Test + DOT Breath Alcohol
This package combines the DOT drug test with a DOT breath alcohol test. Register Now
DOT Drug Test + DOT Physical
This package combines the DOT Physical with the DOT Drug Test. DOT physical exams include a vision and hearing test, blood test, and urinalysis, and is required periodically for workers such as truck drivers. Register Now
DOT Random Pool Membership
Our DOT Random Drug Testing Pool makes compliance easy by allowing Health Street to manage your random pool employee selections. Register Now

Frequently Asked Questions

How long do drug test results take?

Negative urine drug tests typically come back the next business day, but in some cases can take longer. If the drug screening is not negative, further testing is required. Our lab will run a GC/MS confirmation test on all non-negatives, which provides an official, quantitative measurement of the level of drugs in the system. Confirmation testing takes approximately 3-5 days.

Who needs a DOT drug screen?

All transportation industry modalities require DOT drug screening for various purposes. For example, trucking companies must ensure safety-sensitive staff such as drivers are tested on a pre-employment and random basis, as well as for post-accident, reasonable suspicion, return-to-duty, and follow-up testing when indicated.

Who pays for the DOT Drug Test?

DOT regulations are silent about who is responsible for paying for drug testing. Typically, the company pays for pre-employment and random DOT drug tests, but not always. However, if a driver fails a test, smaller companies are much less likely to pay for a driver's return-to-duty and follow-up testing, putting the onus on the driver to pay for the tests.

Large fleets, on the other hand, often pay for all tests, including return-to-duty and follow-up testing, especially if the drivers are unionized.

Is return-to-duty testing required?

Return to duty tests are only required if a person has failed a test, sought out the counsel of a Substance Abuse Professional, and was subsequently cleared to return to duty by the SAP. DOT regulations require trucking firms to ensure that drivers who are subject to the return-to-duty and follow up testing are complying with those testing protocols, including passing all tests as outlined by the Substance Abuse Professional.

Who reviews the DOT drug test results?

DOT drug tests are reviewed by Medical Review Officers (MRO). An MRO signature is required on all valid DOT drug tests, whether positive or negative.

What information does Health Street need so I can send the driver for the test?

To schedule a drug test with Health Street, we will ask for the following information during the registration process:

Company name
Employee name
ZIP code
What happens if I failed a DOT drug test?

If you fail a DOT drug test, please review the information provided by the FMCSA (Federal Motor Carrier Safety Administration) to learn more about the next steps. Health Street is available to assist you in scheduling DOT drug tests as required.

About Our DOT Drug Test

Reasons for Drug Testing

DOT Drug and alcohol tests are administered under the federal authority of the agency overseeing the industry in which a company operates. DOT and its agencies define the reasons which prompt the need for tests. The opposite is also true. DOT drug tests cannot be administered unless it falls into one of the predefined categories. The following are reasons when DOT drug tests must be given:

Post accident
Reasonable suspicion
Return to duty
Follow up
Reviewing Drug Test Results

The result of a DOT Drug Test is reported in a secure PDF file which details the list of the tested drugs. For each class of drugs, there is an indication of positive or negative. If a drug comes back positive, the specific metabolite which tested positive will be indicated. The PDF file can be easily downloaded from our portal for printing or forwarding.

Why Choose Health Street

SAMHSA Certified Laboratory and MRO

Like all of our lab based tests, DOT Drug Tests are processed at a SAMHSA Certified laboratory. Results are reviewed by one of our Medical Review Officers (MRO). Our MROs are licensed physicians and toxicology experts. The MRO will also release the final results, which will be reported to the employer or person who requested the test.

StaffGlass™... the Employer eResults Portal

Business owners get free access to our secure Employer eResults Portal, StaffGlass, to place drug test orders, view test results, manage employee screenings and eSignature documents (such as policies and procedures), update random drug testing pools, and register for background checks. Non-business clients get results via email and/or fax.

What Drugs Does a DOT Drug Test Cover?

The following substances can be detected in our DOT Drug Test. Use the below links for more information about each substance:

arrow_right_alt Cocaine
arrow_right_alt Angel Dust (PCP)
arrow_right_alt Morphine
arrow_right_alt Codeine
arrow_right_alt Heroin
arrow_right_alt Amphetamines
arrow_right_alt Methamphetamines
arrow_right_alt Ecstasy
arrow_right_alt Hydrocodone
arrow_right_alt Oxycodone

Related Tests

If you'd like to see a comprehensive list of all DOT tests that Health Street offers, you can view all DOT tests and services here.

If a DOT Drug Test is not what you need, try taking a look at our DOT Breath Alcohol Test or our DOT Random Pool. We also offer DOT Supervisor Training.


Excellent Health Street Communication
Star Rating
By Hanna S. on 4/11/2024
Bowen Trucking
We were told by Health Street that we could go in for testing 10am-3pm. Get to lab corp for testing and we were told that they don’t do testing 11:30am to 1:30 pm. Very frustrating as we arrived at 11:32 am. Other than that, the communication from Health Street has been excellent.
Three Times Charm
Star Rating
By Robert M. on 2/22/2022
Byram Farms Transportation LLC
Went three times until someone was there to do the test
Excellent Timely Service
Star Rating
By Nelson D. on 2/18/2022
I submitted a urine sample 24 hours ago for my drug test for my USCG Captain's License. I have just received the results. Excellent communications, timely service. Thanks. Nelson Corbin
Very Helpful Ryan
Star Rating
By Phillip R. on 2/18/2022
Ryan was very helpful.I think he deserves a good pay raise.
Professional, Friendly
Star Rating
By Gregory on 2/11/2022
Professional and friendly.
Quick, Professional
Star Rating
By Winston J. on 2/7/2022
Quick and professional
Amazing Service
Star Rating
By Shamsiddin T. on 2/3/2022
Prompt Service
Star Rating
By Norman S. on 1/18/2022
Booker Trans
A.Service was prompt.B.St. Joseph, Mo. C.DOT Post Accident Drug & Alcohol test
Guys Awesome
Star Rating
By John F. on 1/16/2022
Cyclone Septic Services
You guys are awesome
Great Place
Star Rating
By Chris on 1/11/2022
Great place