DOT Drug Test - info-hero

DOT Drug Test

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Whether you're hiring CDL drivers or workers for trucking, shipping, aviation, rail, transit, or merchant marine roles, DOT drug testing is required for safety-sensitive employees—and it's not something you want to get wrong. From pre-employment drug tests to random drug testing pools, Health Street makes it easy to stay in compliance with DOT regulations.

With fast scheduling and thousands of DOT drug testing sites near you, we serve both employers and individuals who need to meet federal transportation drug testing requirements.

Wondering how much a DOT drug test costs, where to get a DOT drug test, or how long a DOT drug test takes? This page covers everything you need to know. Register online or call (888) 378-2499 to schedule a DOT drug screen near you.

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Frequently Asked Questions

How long does it take for a DOT drug test to come back?

The DOT pre-employment drug test time frame is fast. Negative urine specimens typically come back the next business day, but in some cases can take longer. If the drug screening is not negative, further testing is required. Our lab will run a GC/MS confirmation test on all non-negatives urine samples, which provides an official, quantitative measurement of the level of drugs in the system. Confirmation testing takes approximately 3-5 days.

Why is my DOT drug test taking so long?

Most negative DOT drug test results are returned within one business day. However, delays can happen for a few reasons. If the test is non-negative, it must go through a more detailed GC/MS confirmation process, which can take an additional 3–5 days. Delays can also occur because of issues like incomplete paperwork, specimen temperature concerns, or if the Medical Review Officer (MRO) needs to contact the employee for more information, such as verifying prescriptions.

Who needs a DOT drug screen?

All transportation industry modalities require DOT drug screening for various purposes. For example, trucking companies must ensure that safety sensitive positions, such as drivers, are tested on a pre-employment and random basis, as well as for post-accident, reasonable suspicion, return-to-duty, and follow-up testing when indicated.

Who is responsible for paying for the test?

DOT regulations are silent about who is responsible for paying for drug testing. Typically, the drug testing for trucking companies is covered by the company, but not always. However, if a driver fails a test, smaller companies are much less likely to pay for a driver's return-to-duty and follow-up testing, putting the onus on the driver to pay for the tests.

Large fleets, on the other hand, often pay for all logistics drug testing, including return-to-duty and follow-up testing, especially if the drivers are unionized.

Is return-to-duty testing required?

Return to duty tests are only required if a person has failed a test, sought out the counsel of a Substance Abuse Professional, and was subsequently cleared to return to duty by the SAP. DOT regulations require trucking firms to ensure that drivers who are subject to the return to duty process and follow up testing are complying with those testing protocols, including passing all tests as outlined by the Substance Abuse Professional (SAP).

Who reviews the drug test results?

Drug tests are reviewed by Medical Review Officers (MRO). An MRO signature is required on all valid DOT tests, whether positive or negative.

What happens if you fail a DOT physical drug test?

If you fail a DOT drug screen, please review the information provided by the FMCSA (Federal Motor Carrier Safety Administration) to learn more about the next steps. Health Street is available to assist you in scheduling DOT drug screens as required.

What is a DOT Physical?

A DOT physical is a medical exam required for certain workers, like truck drivers, to ensure they meet health and safety standards. It includes vision and hearing tests, a blood test, and a urinalysis. Some packages also combine the DOT physical with a drug test.

Can I take an at-home drug test for DOT-regulated positions?

No. DOT testing is federally regulated, and you cannot take an at-home drug test to fulfill the requirements. Under 49 CFR Part 40, the U.S. Department of Transportation (DOT) only authorizes urine and oral fluid specimens for drug testing, and only when collected under specific procedures at certified collection sites. These specimens must be screened and confirmed at HHS-certified laboratories.

What information does Health Street need so I can send the driver for the test?

To schedule a drug test with Health Street, we will ask for the following information during the registration process:

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Company name
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Employee name
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ZIP code

About Our DOT Drug Test

Health Street offers DOT-compliant drug testing for truck drivers, pilots, rail workers, maritime employees, and others in transportation and logistics. We provide easy access to trucking drug testing facilities near you, so you can schedule with just a few clicks.

Reasons for Drug Testing

DOT drug and alcohol testing programs are administered under the federal authority of the agency overseeing the industry in which a company operates. DOT and its agencies define the reasons that prompt the need for tests. The opposite is also true: DOT drug screens cannot be administered unless they fall into one of the predefined categories. The following are reasons when DOT drug screenings must be given:

  • Pre-employment DOT drug tests
  • Random testing through a DOT consortium or company pool
  • Post-accident drug screens
  • Reasonable suspicion testing
  • Return-to-duty and follow-up DOT testing

Reviewing Drug Test Results

The result of a drug test is reported in a secure PDF file which details the list of the tested drugs. For each class of drugs, there is an indication of positive or negative. If a drug comes back positive, the specific metabolite which tested positive will be indicated. The PDF file can be easily downloaded from our portal for printing or forwarding.

Why Choose Health Street

Not all DOT drug testing companies put your needs first, but we do. At Health Street, we offer:

  • Safe, secure, and fast drug testing with accurate results from licensed labs
  • Nationwide network of more than 10,000 DOT drug test locations
  • Mobile drug testing available for companies and individuals
  • Secure portal for easy test management and viewing of results
  • Trusted provider in the drug testing industry

What Drugs Does the Urine DOT Drug Test Cover?

The following substances can be detected in our DOT drug screens. Use the links below for more information about each substance:

Related Tests

If you'd like to see a comprehensive list of all DOT tests that Health Street offers, you can view all DOT tests and services here.

If you're looking for something else, try our DOT Breath Alcohol Test or our DOT Random Pool. We also offer DOT Supervisor Training.

DOT Compliance Resources

Below are some resources to learn more about compliance for the Department of Transportation (DOT), Federal Motor Carrier Safety Administration (FMCSA), United States Coast Guard (USCG), Federal Aviation Administration (FAA), and Pipeline and Hazardous Materials Safety Administration (PHMSA):

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