DOT Drug Test - info-hero

DOT Drug Test

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A DOT Drug Test is for truckers, pilots, captains, and safety-sensitive employees governed by DOT regulations in all transportation modalities. Pre-employment, Random, Reasonable Suspicon & Post-Accident testing are available, as well as Return to Duty and Follow-Up testing. Employers and individuals can find a location for a DOT drug test and register online, or call (888) 378-2499 to set up testing for staff or others. We offer DOT drug testing services at over 10,000 clinic locations nationwide.

Our online registration process is simple. You choose the lab location based on ZIP code during registration. An authorization barcode is instantly emailed to you and texted directly to the phone of the person being tested. A map of the clinic location will accompany the barcode. The registrant can then walk in to the drug testing facility and show the barcode along with photo ID. Results are fast, signed by a doctor, and reported securely online.

Workforce Screening, Digitally Delivered

Karen Mintz
I am using Health Street to assist me with DOT drug testing requirements. I love it!!!!!!! Strait forward and the Tech support has been great.
Charles Pinotti
Dun Rite Sand and Gravel Co Inc.
Appreciate the professional and efficient response.
Lisa Hood
Ruff Dimensional Stone LLC
Great service, quick results, excellent staff.
Thomas A Collins Jr
Star Rating
4.9 out of 5 stars (415 reviews)

DOT Drug Test Options

DOT Drug Test
Health Street’s DOT Drug Test complies with DOT regulations for drug testing of safety-sensitive workers in transportation industries, such as trucking, shipping, aviation, piplelines and hazardous materials. Find Clinic Now
DOT Drug Test + DOT Breath Alcohol
This package combines the DOT drug test with a DOT breath alcohol test. Find Clinic Now
DOT Drug Test + DOT Physical
This package combines the DOT Physical with the DOT Drug Test. DOT physical exams include a vision and hearing test, blood test, and urinalysis, and is required periodically for workers such as truck drivers. Find Clinic Now
DOT Random Pool Membership
Our DOT Random Drug Testing Pool makes compliance easy by allowing Health Street to manage your random pool employee selections. Find Clinic Now

Frequently Asked Questions

How Long Do Drug Test Results Take?

Negative urine drug tests typically come back the next business day, but in some cases can take longer. If the drug screening is not negative, further testing is required. Our lab will run a GC/MS confirmation test on all non-negatives, which provides an official, quantitative measurement of the level of drugs in the system. Confirmation testing takes approximately 3-5 days.

Who Needs a DOT Drug Screen?

All transportation industry modalities require DOT drug screening for various purposes. For example, trucking companies must ensure safety-sensitive staff such as drivers are tested on a pre-employment and random basis, as well as for post-accident, reasonable suspicion, return-to-duty, and follow-up testing when indicated.

Who Pays for the DOT Drug Test?

DOT regulations are silent about who is responsible for paying for drug testing. Typically, the company pays for pre-employment and random DOT drug tests, but not always. However, if a driver fails a test, smaller companies are much less likely to pay for a driver’s return-to-duty and follow-up testing, putting the onus on the driver to pay for the tests.

Large fleets, on the other hand, often pay for all tests, including return-to-duty and follow-up testing, especially if the drivers are unionized.

Is Return-to-Duty Testing Required?

Return to duty tests are only required if a person has failed a test, sought out the counsel of a Substance Abuse Professional, and was subsequently cleared to return to duty by the SAP. DOT regulations require trucking firms to ensure that drivers who are subject to the return-to-duty and follow up testing are complying with those testing protocols, including passing all tests as outlined by the Substance Abuse Professional.

Who Reviews the DOT Drug Test Results?

DOT drug tests are reviewed by Medical Review Officers (MRO). An MRO signature is required on all valid DOT drug tests, whether positive or negative.

About Our DOT Drug Test

Reasons for Drug Testing

DOT Drug and alcohol tests are administered under the federal authority of the agency overseeing the industry in which a company operates. DOT and its agencies define the reasons which prompt the need for tests. The opposite is also true. DOT drug tests cannot be administered unless it falls into one of the predefined categories. The following are reasons when DOT drug tests must be given:

Post accident
Reasonable suspicion
Return to duty
Follow up
Reviewing Drug Test Results

The result of a DOT Drug Test is reported in a secure PDF file which details the list of the tested drugs. For each class of drugs, there is an indication of positive or negative. If a drug comes back positive, the specific metabolite which tested positive will be indicated. The PDF file can be easily downloaded from our portal for printing or forwarding.

Why Choose Health Street

SAMHSA Certified Laboratory and MRO

Like all of our lab based tests, DOT Drug Tests are processed at a SAMHSA Certified laboratory. Results are reviewed by one of our Medical Review Officers (MRO). Our MROs are licensed physicians and toxicology experts. The MRO will also release the final results, which will be reported to the employer or person who requested the test.

StaffGlass™… the Employer eResults Portal

Business owners get free access to our secure Employer eResults Portal, StaffGlass, to place drug test orders, view test results, manage employee screenings and eSignature documents (such as policies and procedures), update random drug testing pools, and register for background checks. Non-business clients get results via email and/or fax.

What Drugs Does a DOT Drug Test Cover?

The following substances can be detected in our DOT Drug Test. Use the below links for more information about each substance:

arrow_right_alt Cocaine
arrow_right_alt Angel Dust (PCP)
arrow_right_alt Morphine
arrow_right_alt Codeine
arrow_right_alt Heroin
arrow_right_alt Amphetamines
arrow_right_alt Methamphetamines
arrow_right_alt Ecstasy
arrow_right_alt Hydrocodone
arrow_right_alt Oxycodone

Related Tests

If you’d like to see a comprehensive list of all DOT tests that Health Street offers, you can view all DOT tests and services here.

If a DOT Drug Test is not what you need, try taking a look at our DOT Breath Alcohol Test or our DOT Random Pool. We also offer DOT Supervisor Training.


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By Rene M on 8/15/2021
The staff is very friendly.,good job.
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By Lorienzo Garner on 8/14/2021
Very Professional and experienced group. Fast and simple to the point.
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By Angel sanchez on 7/27/2021
Everything was okay
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By Lynn Humphreys on 7/21/2021
Jim Thorpe Trolly Company
Such a great service- makes everything easy to manage!
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By Jeffery McCameron on 7/9/2021
You were good upon response and follow up
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By Wilton on 6/22/2021
Like it customer service was great they were helpful treat me good and respectful thankful
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By Phillip on 6/12/2021
Top shelf customer service. If you have a question and call someone will pick up right away. Request and processing online is instant. No waiting involved. Excellent.
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By Joyce on 6/4/2021
Sunshine Bus Inc
customer service was very good and test results came back next day.thank you.
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By Juan Verduzco Jr on 5/7/2021
Very responsive I like the that results are quick thank you for the help
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By Al Santamaria on 4/27/2021
First time using this service. Excellent customer service & communication.