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DOT Drug Test

A DOT Drug Test is for truckers, pilots, captains, and safety-sensitive employees. Pre-employment, Random, & Post-Accident testing is available. Employers and individuals can find a location for a DOT drug test and register online, or call (888) 378-2499 to set up testing for staff or others. We offer DOT drug testing services at over 5000 clinic locations nationwide.

Our online registration process is simple. You choose the lab location based on ZIP code during registration. An authorization barcode is instantly emailed to you and texted directly to the phone of the person being tested. A map of the clinic location will accompany the barcode. The registrant can then walk in to the drug testing facility and show the barcode along with photo ID. Results are fast, signed by a doctor, and reported securely online.

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4.9 out of 5 stars (412 reviews)
$79 for Random Pool Members
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Fantastic service! Very fast, efficient, and easy. They made finding locations for my employees a snap, and Kept me informed of every status change in the process. Our company will DEFINITELY be using Health Street again.
By Rick Woosley on August 18, 2017
Health Street was SO HELPFUL - I needed immediate assistance since I had a candidate waiting at a facility for a DOT test and needed to set up and make a payment asap. Because of their assistance, she did not need to reschedule her appointment. Very much appreciated!
By Nicole on May 16, 2016
Logo of Health Street's client, Barnes Aerospace
Easy, efficient and the staff was very helpful and great to talk with. Very approachable. This was a very smooth move and we are excited that we have you as a resource. Super easy to maneuver. Straight forward, very refreshing. No issues. Every aspect of this was convenient and very quick and easy. Good people, good software, no stress and quick results. Win, Win!
By Kerry on November 14, 2019
Outwest Express
Outwest Express's Logo
4.9 out of 5 stars (408 reviews)

About DOT Drug Test

List of drugs detected by DOT Drug Test

Reasons for Drug Testing

DOT Drug and alcohol tests are administered under the federal authority of the agency overseeing the industry in which a company operates. DOT and its agencies define the reasons which prompt the need for tests. The opposite is also true. DOT drug tests cannot be administered unless it falls into one of the pre-defined categories. The following are reasons when DOT drug tests must be given:
  • Pre-employment
  • Random
  • Post Accident
  • Reasonable Suspicion
  • Return to duty
  • Follow up

SAMHSA Certified Laboratory and MRO

Like all of our lab based tests, DOT Drug Tests are processed at a SAMHSA Certified laboratory. Results are reviewed by one of our Medical Review Officers (MRO). Our MROs are licensed physicians and toxicology experts. If the result of a urine drug test is non-negative, the MRO will reach out to the person to ask about prescription medications and, if appropriate, to request proof. Once that conversation with the doctor takes place, and the person has a chance to provide proof of any prescriptions, the MRO will release the final results, which will be reported to the Employer or person who requested the test.

Reviewing Drug Test Results

The result of a DOT Drug Test is reported in a secure PDF files which detail the list of the tested drugs. For each class of drugs, there is an indication of Positive or Negative. If a drug comes back positive, the specific metabolite which tested positive will be specifically indicated. Quantitative levels for positive results are available upon request. The PDF file can be easily downloaded from our portal, printed, or forwarded. We are also happy to send or fax drug test results to any person, employer, or organization that needs to get a copy of the report.

How long do drug test results take?

Negative urine drug tests typically come back the next business day, but in some cases can take longer. If the drug screening is not negative, further testing is required. Our lab will run a GC/MS confirmation test on all non-negatives, which provides an official, quantitative measurement of the level of drugs in the system. Confirmation testing takes approximately 3-5 days.

Staff Glass™... the Employer eResults Portal

Business owners get free access to our secure Employer eResults Portal, StaffGlass, to place drug test orders, view test results, manage employee screenings and eSignature documents (such as policies and procedures), update random drug testing pools, and register for background checks. Non-business clients get results via email and/or fax.

How it Works

  • Registration booklet with penRegister Online or By Phone
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  • Patient seated next to clinic practitionerVisit a Health Street Clinic
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  • Magnifying glass over computer screen inspecting checkmarkGet Your Fast, Accurate Results


412 Ratings
4.9 out of 5 stars.
DOT Drug Test
By Angel sanchez on July 27, 2021
Everything was okay
DOT Drug Test
By Lynn Humphreys on July 21, 2021
Jim Thorpe Trolly Company
Such a great service- makes everything easy to manage!
DOT Drug Test
By Jeffery McCameron on July 9, 2021
You were good upon response and follow up
DOT Drug Test
By Wilton on June 22, 2021
Like it customer service was great they were helpful treat me good and respectful thankful
DOT Drug Test
By Phillip on June 12, 2021
Top shelf customer service. If you have a question and call someone will pick up right away. Request and processing online is instant. No waiting involved. Excellent.
DOT Drug Test
By joyce on June 4, 2021
Sunshine Bus Inc
customer service was very good and test results came back next day.thank you.
DOT Drug Test
By Juan Verduzco Jr on May 7, 2021
Very responsive I like the that results are quick thank you for the help
DOT Drug Test
By Al Santamaria on April 27, 2021
First time using this service. Excellent customer service & communication.
DOT Drug Test
By Melissa Jensen on April 1, 2021
Gray Transportation Waterloo
I had to change locations and the customer service rep was polite and got done immediately. The results were also very timely and my employee started on time. Thank you
DOT Drug Test
By Edward Paul Combs on March 31, 2021
Mid America Milling Co. LLC
Its was easy to use and set up. Only took a few minutes of time. Thanks

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