DOT Drug Test - info-hero

DOT Drug Test

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A DOT Drug Test is for truckers, pilots, captains, and safety-sensitive employees governed by DOT regulations in all transportation modalities. Pre-employment, Random, Reasonable Suspicon & Post-Accident testing are available, as well as Return to Duty and Follow-Up testing. Employers and individuals can find a location for a DOT drug test and register online, or call (888) 378-2499 to set up testing for staff or others. We offer DOT drug testing services at over 10,000 clinic locations nationwide.

Our online registration process is simple. You choose the lab location based on ZIP code during registration. An authorization barcode is instantly emailed to you and texted directly to the phone of the person being tested. A map of the clinic location will accompany the barcode. The registrant can then walk in to the drug testing facility and show the barcode along with photo ID. Results are fast, signed by a doctor, and reported securely online.

Workforce Screening, Digitally Delivered

Went three times until someone was there to do the test
Robert M Byram
Byram Farms Transportation LLC
Ryan was very helpful.I think he deserves a good pay raise.
Phillip Rissell
I submitted a urine sample 24 hours ago for my drug test for my USCG Captain’s License. I have just received the results. Excellent communications, timely service. Thanks. Nelson Corbin
Nelson D Corbin
Professional and friendly.
Star Rating
4.8 out of 5 stars (445 reviews)

DOT Drug Test Options

DOT Drug Test
Health Street’s DOT Drug Test complies with DOT regulations for drug testing of safety-sensitive workers in transportation industries, such as trucking, shipping, aviation, piplelines and hazardous materials. Register Now
DOT Drug Test + DOT Breath Alcohol
This package combines the DOT drug test with a DOT breath alcohol test. Register Now
DOT Drug Test + DOT Physical
This package combines the DOT Physical with the DOT Drug Test. DOT physical exams include a vision and hearing test, blood test, and urinalysis, and is required periodically for workers such as truck drivers. Register Now
DOT Random Pool Membership
Our DOT Random Drug Testing Pool makes compliance easy by allowing Health Street to manage your random pool employee selections. Register Now

Frequently Asked Questions

How Long Do Drug Test Results Take?

Negative urine drug tests typically come back the next business day, but in some cases can take longer. If the drug screening is not negative, further testing is required. Our lab will run a GC/MS confirmation test on all non-negatives, which provides an official, quantitative measurement of the level of drugs in the system. Confirmation testing takes approximately 3-5 days.

Who Needs a DOT Drug Screen?

All transportation industry modalities require DOT drug screening for various purposes. For example, trucking companies must ensure safety-sensitive staff such as drivers are tested on a pre-employment and random basis, as well as for post-accident, reasonable suspicion, return-to-duty, and follow-up testing when indicated.

Who Pays for the DOT Drug Test?

DOT regulations are silent about who is responsible for paying for drug testing. Typically, the company pays for pre-employment and random DOT drug tests, but not always. However, if a driver fails a test, smaller companies are much less likely to pay for a driver’s return-to-duty and follow-up testing, putting the onus on the driver to pay for the tests.

Large fleets, on the other hand, often pay for all tests, including return-to-duty and follow-up testing, especially if the drivers are unionized.

Is Return-to-Duty Testing Required?

Return to duty tests are only required if a person has failed a test, sought out the counsel of a Substance Abuse Professional, and was subsequently cleared to return to duty by the SAP. DOT regulations require trucking firms to ensure that drivers who are subject to the return-to-duty and follow up testing are complying with those testing protocols, including passing all tests as outlined by the Substance Abuse Professional.

Who Reviews the DOT Drug Test Results?

DOT drug tests are reviewed by Medical Review Officers (MRO). An MRO signature is required on all valid DOT drug tests, whether positive or negative.

About Our DOT Drug Test

Reasons for Drug Testing

DOT Drug and alcohol tests are administered under the federal authority of the agency overseeing the industry in which a company operates. DOT and its agencies define the reasons which prompt the need for tests. The opposite is also true. DOT drug tests cannot be administered unless it falls into one of the predefined categories. The following are reasons when DOT drug tests must be given:

Post accident
Reasonable suspicion
Return to duty
Follow up
Reviewing Drug Test Results

The result of a DOT Drug Test is reported in a secure PDF file which details the list of the tested drugs. For each class of drugs, there is an indication of positive or negative. If a drug comes back positive, the specific metabolite which tested positive will be indicated. The PDF file can be easily downloaded from our portal for printing or forwarding.

Why Choose Health Street

SAMHSA Certified Laboratory and MRO

Like all of our lab based tests, DOT Drug Tests are processed at a SAMHSA Certified laboratory. Results are reviewed by one of our Medical Review Officers (MRO). Our MROs are licensed physicians and toxicology experts. The MRO will also release the final results, which will be reported to the employer or person who requested the test.

StaffGlass™… the Employer eResults Portal

Business owners get free access to our secure Employer eResults Portal, StaffGlass, to place drug test orders, view test results, manage employee screenings and eSignature documents (such as policies and procedures), update random drug testing pools, and register for background checks. Non-business clients get results via email and/or fax.

What Drugs Does a DOT Drug Test Cover?

The following substances can be detected in our DOT Drug Test. Use the below links for more information about each substance:

arrow_right_alt Cocaine
arrow_right_alt Angel Dust (PCP)
arrow_right_alt Morphine
arrow_right_alt Codeine
arrow_right_alt Heroin
arrow_right_alt Amphetamines
arrow_right_alt Methamphetamines
arrow_right_alt Ecstasy
arrow_right_alt Hydrocodone
arrow_right_alt Oxycodone

Related Tests

If you’d like to see a comprehensive list of all DOT tests that Health Street offers, you can view all DOT tests and services here.

If a DOT Drug Test is not what you need, try taking a look at our DOT Breath Alcohol Test or our DOT Random Pool. We also offer DOT Supervisor Training.


Star Rating
By Winston Jorgensen on 2/7/2022
Quick and professional
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By William Wicks on 12/25/2021
Thank you for the very professional services. Merry Christmas & Happy New Year!
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By Frank Martinez on 12/21/2021
Customer service was awesome and great.I had my service done in Bakersfield California. I ordered a DOT RTD drug test.
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By Miranda Robinson on 12/17/2021
C&W Global
Usually pretty good. Lately results have been taking a very long time.
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By Sommer Alexander on 12/13/2021
JFS Logistics
Our business recently started using this company and so far I can say we have had great customer service and professional interactions with our employee screening needs.
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By Eric on 12/8/2021
Your system great
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By Eric on 12/8/2021
Awesome!!!! Great system
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By Bradley Carpenter on 11/19/2021
Great service
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By Jacqueline Goins on 11/9/2021
Ease of use and quick results. We are very happy partnering with Health Street for our screenings.
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By Quinshard on 11/3/2021
Phenomenal service. I was able to get my DOT Physical taken care of quick and easy.