Drug Testing in Orlando, Florida

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Health Street provides drug and alcohol testing in Orlando, Florida at 26 conveniently located drug testing centers. Call (407) 519-2581 or register online. Drug testing options include DOT and non-DOT urine drug tests, breath alcohol tests, EtG alcohol tests, and hair drug testing.

Whether you’re drug testing your employees, yourself, or a family member, just choose your test and pick a laboratory testing location. Rest assured, a registration barcode will be sent to you immediately (or to the person you specify). A map and GPS directions will be linked to the barcode. Test results are fast, accurate, and reported securely in our online portal.

Workforce Screening, Digitally Delivered

I went to the site on Orange Avenue near Michigan Avenue in Orlando, FL. I ordered the 5 panel drug test. Customer service was great.
Krinundi Ward
9/20/2021
Very easy to use
Robert Dussault
5/12/2021
Very easy to use
Robert Dussault
5/12/2021
Always, always 100 professional, accurate and super quick with results!
Tandy Gilstrap
11/20/2020
Quick easy registration, next day walk in. Results back next day! Awesome service!!!
Tandy Gilstrap
8/1/2020
Star Rating
5 out of 5 stars (12 reviews)
1 of 26
2200 N Alafaya Trl
Orlando, FL 32826
2 of 26
4401 S Orange Avenue
Orlando, FL 32806
3 of 26
5425 S Semoran Blvd
Orlando, FL 32822
4 of 26
7300 Sandlake Commons Blvd
Orlando, FL 32819
5 of 26
2504 S Alafaya Trl
Orlando, FL 32828
6 of 26
1603 S Hiawassee Road
Orlando, FL 32835
7 of 26
14050 Town Loop Blvd
Orlando, FL 32837
8 of 26
114 Ernestine Street
Orlando, FL 32801
9 of 26
4632 36th Street
Orlando, FL 32811
10 of 26
7780 Lake Underhill Road
Orlando, FL 32822
11 of 26
1430 N Mills Avenue
Orlando, FL 32803
12 of 26
626 N Thornton Avenue
Orlando, FL 32803
13 of 26
7751 Kingspointe Parkway
Orlando, FL 32819
14 of 26
2555 S Kirkman Road
Orlando, FL 32811
15 of 26
2323 S Orange Avenue
Orlando, FL 32806
16 of 26
4780 N Orange Blossom Trl
Orlando, FL 32810
17 of 26
8119 S Orange Avenue
Orlando, FL 32809
18 of 26
12421 South Orange Blossom Trail
Orlando, FL 32837
19 of 26
3101 W Princeton Street
Orlando, FL 32808
20 of 26
7300 Sandlake Commons Blvd 205
Orlando, FL 32819
21 of 26
8865 Commodity Cir 13-216
Orlando, FL 32819
22 of 26
2415 W Sand Lake Road
Orlando, FL 32809
23 of 26
311 S Avalon Park Blvd
Orlando, FL 32829
24 of 26
8972 Turkey Lake Road
Orlando, FL 32819
25 of 26
13935 Landstar Blvd
Orlando, FL 32824
26 of 26
2438 S Kirkman Road
Orlando, FL 32811
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Drug Testing in Orlando, Florida

Orlando FL Drug Testing Locations

Get Drug Testing in Orlando, Florida Right Now

Health Street has the largest selection of drug testing and employment screening services in Orlando. Employers can access our services to set up a drug free workplace, families can get court ordered drug testing, and attorneys and private individuals can register for non-DOT drug tests, Department of Transportation (DOT) drug tests, and more.

Available Drug Testing Methods

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Learn about our Hair Follicle Drug Tests
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See a full list of our Urine Drug Testing services.

Popular Drug Testing Panels

Specialized Categories of Tests

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For companies that don’t wish to include marijuana, we have a slate of drug tests without marijuana
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Choose from our Alcohol Testing options (such as breathalyzer, EtG test, or PEth blood alcohol)
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Read more about our Court Ordered Drug Testing for individuals and families.

DOT Drug Testing

Learn about our DOT drug and alcohol testing services, and how your company can maintain Department of Transportation (DOT) Compliance with 46 CFR Part 40, including:

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Employee Drug Testing: eResults Portal for Employers

Business users get access to the amazing StaffGlass™ portal. When logged in, human resources and other company executives can manage their drug testing program, including:

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Register for drug screenings for reasons such as: pre-employment, random, post-accident, or reasonable suspicion.
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Get fast, accurate results in a safe and secure online portal.
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Find a drug test clinic in Orlando, FL or pick from our lab testing locations anywhere in the United States; you can order your test online, view results, and check status of all tests
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Set up State Background Check and employment background screening
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Register staff for Occupational Health Services
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Manage eSign documents related to the testing process
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Create an employee pool for random drug testing, add or remove staff, update staff employment status.
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StaffGlass™ is backed up by world class customer service.
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Call Center Hours: Monday through Friday: 7:30 AM to 7:00 PM Eastern Time.

Why Health Street

A Decade Plus of Experience

Health Street has provided drug tests since 2010. The first time we performed drug testing in Orlando, FL was in 2013.  From then until now, we have completed 884 drug tests at our Orlando labs. 

Dedicated to Excellence

We are dedicated to providing a level of excellence in drug testing in Orlando that is unmatched. 

Orlando, Florida – Drug Testing

Substance abuse statistics for Orlando, FL

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Substance abuse and death rate due to overdoses in Florida are 23.19 percent above the national average
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Annually, fatal drug overdoses cause about 5,268 deaths in Florida
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According to FDOH (Florida Department of Health) data, the number of deadly overdoses in the state in 2020 increased by 37 percent compared to the number of deaths in 2019
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The death rate in Florida is 25.5 deaths out of every 100,000 residents

Orlando, FL: Drug abuse trends

According to the latest data, the opioid epidemic was an even more significant issue than ever during the COVID-19 pandemic. People were isolated and depressed, many struggled with anxiety, and it was harder to get professional help or to socialize regularly. Experts speculate that the pandemic triggered an influx in drug abuse and overdose deaths, including fatal overdoses caused by fentanyl.

The National Center for Health Statistics stated that drug overdoses have increased by more than thirty percent from September 2020 to February 2021. In the year 2020, the number of fatal overdoses increased by 37 percent. Of course, this has led to a significant increase in drug-involved emergency department visits.

Local initiatives to curb drug abuse in Orlando, Florida

Florida is attempting to curb the opioid epidemic through multiple initiatives. In 2019, a new initiative launched in Central Florida called Project Opioid, which plans to take a different approach to solve the opioid crisis. This consortium focuses on community awareness and education to unite people in their efforts to reduce opioid abuse and addiction.

The Florida Courts Opioids and Stimulants Response is another initiative responding to the state’s drug abuse and addiction numbers. This initiative focuses largely on the opioid problems in the state, and stimulants were added in 2020 to address issues of cocaine and methamphetamine. The initiative hosts training events throughout the year and spreads awareness of drug epidemics primarily through courts for problem-solving and families.

Last updated 7/20/2022

Locations

Clinic Hours and Information for our Labs in Orlando, Florida

2200 N Alafaya TrlSuite 900Orlando, FL 32826
Pre-registration required,Walk-in with barcode:

Monday: 8:00 AM – 2:00 PMTuesday: 8:00 AM – 2:00 PMWednesday: 8:00 AM – 2:00 PMThursday: 8:00 AM – 2:00 PMFriday: 8:00 AM – 2:00 PM About this Location: Our clinic is just a few blocks from Lake Rouse.
4401 S Orange AvenueSuite 110Orlando, FL 32806
Pre-registration required,Walk-in with barcode:

Monday: 6:30 AM – 3:00 PMTuesday: 6:30 AM – 3:00 PMWednesday: 6:30 AM – 3:00 PMThursday: 6:30 AM – 3:00 PMFriday: 6:30 AM – 3:00 PM About this Location: It is simple to locate our lab. We are a short walk from Cypress Grove Park.
5425 S Semoran BlvdSuite 7aOrlando, FL 32822
Pre-registration required,Walk-in with barcode:

Monday: 8:00 AM – 2:30 PMTuesday: 8:00 AM – 2:30 PMWednesday: 8:00 AM – 2:30 PMThursday: 8:00 AM – 2:30 PMFriday: 8:00 AM – 2:30 PM About this Location: You can locate our clinic down the block from Mc Coy Elementary School.
7300 Sandlake Commons BlvdSuite 205Orlando, FL 32819
Pre-registration required,Walk-in with barcode:

Monday: 8:00 AM – 2:30 PMTuesday: 8:00 AM – 2:30 PMWednesday: 8:00 AM – 2:30 PMThursday: 8:00 AM – 2:30 PMFriday: 8:00 AM – 2:30 PM About this Location: Our lab is located around the corner from Orange County Convention Center.
2504 S Alafaya TrlSuite 160Orlando, FL 32828
Pre-registration required,Walk-in with barcode:

Monday: 7:30 AM – 3:00 PMTuesday: 7:30 AM – 3:00 PMWednesday: 7:30 AM – 3:00 PMThursday: 7:30 AM – 3:00 PMFriday: 7:30 AM – 3:00 PM About this Location: It is simple to locate our lab. We are very close by to Anytime Fitness.
1603 S Hiawassee RoadSuite 120Orlando, FL 32835
Pre-registration required,Walk-in with barcode:

Monday: 6:30 AM – 3:30 PMTuesday: 6:30 AM – 3:30 PMWednesday: 6:30 AM – 3:30 PMThursday: 6:30 AM – 3:30 PMFriday: 6:30 AM – 3:30 PM About this Location: This office is conveniently located a short walk from MetroWest Golf Club.
14050 Town Loop BlvdSuite 105Orlando, FL 32837
Pre-registration required,Walk-in with barcode:

Monday: 6:00 AM – 3:00 PMTuesday: 6:00 AM – 3:00 PMWednesday: 6:00 AM – 3:00 PMThursday: 6:00 AM – 3:00 PMFriday: 6:00 AM – 3:00 PMSaturday: 7:00 AM – 11:00 AM About this Location: You can find us close to Suki Hanna.
114 Ernestine StreetOrlando, FL 32801
Pre-registration required,Walk-in with barcode:

Monday: 8:30 AM – 4:00 PMTuesday: 8:30 AM – 4:00 PMWednesday: 8:30 AM – 4:00 PMThursday: 8:30 AM – 4:00 PMFriday: 8:30 AM – 4:00 PM About this Location: You can locate our clinic very close by to Lake Lucerne.
4632 36th StreetSuite 405Orlando, FL 32811
Pre-registration required,Walk-in with barcode:

Monday: 9:00 AM – 3:30 PM(M Lunch: 12:30-1:30 PM)Tuesday: 9:00 AM – 3:30 PM(T Lunch: 12:30-1:30 PM)Wednesday: 9:00 AM – 3:30 PM(W Lunch: 12:30-1:30 PM)Thursday: 9:00 AM – 3:30 PM(Th Lunch: 12:30-1:30 PM)Friday: 9:00 AM – 3:30 PM(F Lunch: 12:30-1:30 PM) About this Location: You can locate our clinic just a few blocks from Dead Lizard Brewing Company.
7780 Lake Underhill RoadOrlando, FL 32822
Pre-registration required,Walk-in with barcode:

Monday: 7:00 AM – 2:30 PMTuesday: 7:00 AM – 2:30 PMWednesday: 7:00 AM – 2:30 PMThursday: 7:00 AM – 2:30 PMFriday: 7:00 AM – 2:30 PM About this Location: For urine drug testing in Orlando, Florida, you can find us a short walk from AdventHealth East Orlando ER.
1430 N Mills AvenueUnit 140Orlando, FL 32803
Pre-registration required,Walk-in with barcode:

Monday: 6:30 AM – 3:30 PMTuesday: 6:30 AM – 3:30 PMWednesday: 6:30 AM – 3:30 PMThursday: 6:30 AM – 3:30 PMFriday: 6:30 AM – 3:30 PMSaturday: 7:00 AM – 11:00 AM About this Location: Visit us at this location. We are nearby to Ten10 Brewing Company.
626 N Thornton AvenueOrlando, FL 32803
Pre-registration required,Walk-in with barcode:

Monday: 7:00 AM – 4:00 PMTuesday: 9:00 AM – 6:00 PMWednesday: call for hoursThursday: 9:00 AM – 6:00 PMFriday: 7:00 AM – 2:00 PM About this Location: Our lab is located across from Russian Ballet Orlando. You can’t miss us.
7751 Kingspointe ParkwayOrlando, FL 32819
Pre-registration required,Walk-in with barcode:

Monday: 8:00 AM – 5:00 PMTuesday: 8:00 AM – 5:00 PMWednesday: 8:00 AM – 5:00 PMThursday: 8:00 AM – 5:00 PMFriday: 8:00 AM – 5:00 PM About this Location: You can obtain urine and hair drug testing in Orlando, Florida by visiting us at this location. It is a short walk from Shingle Creek Regional Trail.
2555 S Kirkman RoadOrlando, FL 32811
Pre-registration required,Walk-in with barcode:

Monday: 8:00 AM – 5:00 PMTuesday: 8:00 AM – 5:00 PMWednesday: 8:00 AM – 5:00 PMThursday: 8:00 AM – 5:00 PMFriday: 8:00 AM – 5:00 PMSaturday: 8:00 AM – 4:00 PM About this Location: You can locate our clinic just a few blocks from Eagle Nest Park.
2323 S Orange AvenueOrlando, FL 32806
Pre-registration required,Walk-in with barcode:

Monday: 8:00 AM – 5:00 PMTuesday: 8:00 AM – 5:00 PMWednesday: 8:00 AM – 5:00 PMThursday: 8:00 AM – 5:00 PMFriday: 8:00 AM – 5:00 PMSaturday: 8:00 AM – 4:00 PMSunday: 8:00 AM – 4:00 PM About this Location: Visit us at this location. We are just a few blocks from Wadeview Park.
4780 N Orange Blossom TrlOrlando, FL 32810
Pre-registration required,Walk-in with barcode:

Monday: 7:30 AM – 5:00 PMTuesday: 7:30 AM – 5:00 PMWednesday: 7:30 AM – 5:00 PMThursday: 7:30 AM – 5:00 PMFriday: 7:30 AM – 5:00 PM About this Location: We are a short walk from Lake Fairview Park. We look forward to serving you here.
8119 S Orange AvenueSuite 132Orlando, FL 32809
Pre-registration required,Walk-in with barcode:

Monday: 8:00 AM – 5:00 PMTuesday: 8:00 AM – 5:00 PMWednesday: 8:00 AM – 5:00 PMThursday: 8:00 AM – 5:00 PMFriday: 8:00 AM – 5:00 PM About this Location: You can locate our clinic down the block from Southport Community Park.
12421 South Orange Blossom TrailOrlando, FL 32837
Pre-registration required,Walk-in with barcode:

Monday: 7:00 AM – 5:00 PMTuesday: 7:00 AM – 5:00 PMWednesday: 7:00 AM – 5:00 PMThursday: 7:00 AM – 5:00 PMFriday: 7:00 AM – 5:00 PM About this Location: You can find us very close by to Taste of Peru.
3101 W Princeton StreetOrlando, FL 32808
Pre-registration required,Walk-in with barcode:

Monday: 7:00 AM – 3:30 PMTuesday: 7:00 AM – 3:30 PMWednesday: 7:00 AM – 3:30 PMThursday: 7:00 AM – 3:30 PMFriday: 7:00 AM – 3:30 PMSaturday: 7:00 AM – 2:00 PM About this Location: Our lab is located a short walk from Orlando Science Schools – Middle School Campus.
7300 Sandlake Commons Blvd 205Orlando, FL 32819
Pre-registration required,Walk-in with barcode:

Monday: 6:30 AM – 3:00 PMTuesday: 6:30 AM – 3:00 PMWednesday: 6:30 AM – 3:00 PMThursday: 6:30 AM – 3:00 PMFriday: 6:30 AM – 3:00 PM About this Location: We are located just a few blocks from MedPlex Dental. There is plenty of parking available.
8865 Commodity Cir 13-216Orlando, FL 32819
Pre-registration required,Walk-in with barcode:

Monday: 8:30 AM – 4:30 PM(M Lunch: 12:30-2 PM)Tuesday: 8:30 AM – 4:30 PM(T Lunch: 12:30-2 PM)Wednesday: 8:30 AM – 4:30 PM(W Lunch: 12:30-2 PM)Thursday: 8:30 AM – 4:30 PM(Th Lunch: 12:30-2 PM)Friday: 8:30 AM – 4:30 PM(F Lunch: 12:30-2 PM) About this Location: We are located around the corner from OneBlood.
2415 W Sand Lake RoadOrlando, FL 32809
Pre-registration required,Walk-in with barcode:

Monday: 9:00 AM – 4:00 PMTuesday: 9:00 AM – 4:00 PMWednesday: 9:00 AM – 4:00 PMThursday: 9:00 AM – 4:00 PMFriday: 9:00 AM – 4:00 PM About this Location: This office is conveniently located just a few blocks from Lake Ellenor.
311 S Avalon Park BlvdOrlando, FL 32829
Pre-registration required,Walk-in with barcode:

Monday: 8:00 AM – 5:00 PMTuesday: 8:00 AM – 5:00 PMWednesday: 8:00 AM – 5:00 PMThursday: 8:00 AM – 5:00 PMFriday: 8:00 AM – 5:00 PM About this Location: Our clinic is down the block from Bella Vida Park.
8972 Turkey Lake RoadOrlando, FL 32819
Pre-registration required,Walk-in with barcode:

Monday: 8:00 AM – 8:00 PMTuesday: 8:00 AM – 8:00 PMWednesday: 8:00 AM – 8:00 PMThursday: 8:00 AM – 8:00 PMFriday: 8:00 AM – 8:00 PMSaturday: 8:00 AM – 8:00 PMSunday: 8:00 AM – 5:00 PM About this Location: You can locate our clinic a short walk from Little Sand Lake. We hope to see you here soon.
13935 Landstar BlvdOrlando, FL 32824
Pre-registration required,Walk-in with barcode:

Monday: 8:00 AM – 8:00 PMTuesday: 8:00 AM – 8:00 PMWednesday: 8:00 AM – 8:00 PMThursday: 8:00 AM – 8:00 PMFriday: 8:00 AM – 8:00 PMSaturday: 8:00 AM – 8:00 PMSunday: 8:00 AM – 5:00 PM About this Location: It is simple to locate our lab. We are just a few blocks from Meadow Woods Elementary School.
2438 S Kirkman RoadOrlando, FL 32811
Pre-registration required,Walk-in with barcode:

Monday: 8:00 AM – 4:00 PMTuesday: 8:00 AM – 4:00 PMWednesday: 8:00 AM – 4:00 PMThursday: 8:00 AM – 4:00 PMFriday: 8:00 AM – 4:00 PM About this Location: You can locate our clinic down the block from UCF Connect Library – Valencia West.

Some Orlando Neighborhoods That We Serve

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Central Business District
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Lake Nona Region
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Millenia
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Metro West
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Parramore
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Lake Underhill
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Mills 50
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Lake Eola Heights
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Wadeview Park
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Milk District
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Carver Shores
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Lake Nona Medical City

Additional Services

Health Street offers a variety of other services, including:

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Florida Background Check for businesses hiring in Orlando, FL

Local Reviews

Star Rating
By Max Nevin on 3/6/2018
Fast, helpful and detailed. I recieved many detailed texts throughout the 5 panel drug test i ordered and it was a life saver.
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By Charla on 2/20/2018
American Mulch and Groundcover LLC
Prompt reliable service as always. Works well, no downtime. Personalized prompt service
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By Rob on 1/4/2018
Great communication, very professional
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By Daniel Bonilla on 12/1/2017
InProduction
Great customer service. Thanks for the follow up on the Puerto Rico review.
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By Camille Norman on 8/8/2017
Great job! Delenia got exactly what I needed, was very pleasant and professional and got it to me extremely quick! Love it!
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By Jason R Fragale on 5/19/2016
(A) customer service was AAAwesome!!! She is the WO-MAN!!!! Can’t say she is the man because that would be weird. (B) Orlando, Florida. (C) 5-Panel drug test. I would totally be her friend!!!
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By Norbert falasco on 1/8/2016
excellent and patient.

Citations

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Drug overdose death statistics [2022]: Opioids, fentanyl & more. NCDAS. (2022, April 6). Retrieved July 20, 2022, from https://drugabusestatistics.org/drug-overdose-deaths/#oregon
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Statewide Drug Policy Advisory Council 2021 Annual report. (n.d.). Retrieved July 20, 2022, from https://www.floridahealth.gov/%5C/provider-and-partner-resources/dpac/_documents/DPAC_Annual_Report_2021.pdf
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Orange County to lead Central Florida Initiative Combating Opioid Epidemic and Drug Abuse. OCFL Newsroom. (2022, January 12). Retrieved July 20, 2022, from https://newsroom.ocfl.net/2019/02/orange-county-to-lead-central-florida-initiative-combating-opioid-epidemic-and-drug-abuse/
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Opioid & Stimulants initiative. Florida Office of State Courts Administrator. (n.d.). Retrieved July 20, 2022, from https://www.flcourts.org/Resources-Services/Office-of-Problem-Solving-Courts/Opioids-Stimulants-Initiative