Drug Testing in Orlando, Florida

Register Now

Health Street provides drug and alcohol testing in Orlando, Florida at 23 conveniently located drug testing centers. Call (407) 519-2581 or register online. Drug testing options include DOT and non-DOT urine drug tests, breath alcohol tests, EtG alcohol tests, and hair drug testing.

Whether you’re drug testing your employees, yourself, or a family member, just choose your test and pick a laboratory testing location. Rest assured, a registration barcode will be sent to you immediately (or to the person you specify). A map and GPS directions will be linked to the barcode. Test results are fast, accurate, and reported securely in our online portal.

Workforce Screening, Digitally Delivered

I went to the site on Orange Avenue near Michigan Avenue in Orlando, FL. I ordered the 5 panel drug test. Customer service was great.
Krinundi Ward
9/20/2021
Very easy to use
Robert Dussault
5/12/2021
Very easy to use
Robert Dussault
5/12/2021
Always, always 100 professional, accurate and super quick with results!
Tandy Gilstrap
11/20/2020
Quick easy registration, next day walk in. Results back next day! Awesome service!!!
Tandy Gilstrap
8/1/2020
Star Rating
5 out of 5 stars (12 reviews)
1 of 23
2200 N Alafaya Trl
Orlando, FL 32826
2 of 23
4401 S Orange Avenue
Orlando, FL 32806
3 of 23
5425 S Semoran Blvd
Orlando, FL 32822
4 of 23
7300 Sandlake Commons Blvd
Orlando, FL 32819
5 of 23
2504 S Alafaya Trl
Orlando, FL 32828
6 of 23
1603 S Hiawassee Road
Orlando, FL 32835
7 of 23
14050 Town Loop Blvd
Orlando, FL 32837
8 of 23
114 Ernestine Street
Orlando, FL 32801
9 of 23
4632 36th Street
Orlando, FL 32811
10 of 23
7780 Lake Underhill Road
Orlando, FL 32822
11 of 23
1430 N Mills Avenue
Orlando, FL 32803
12 of 23
626 N Thornton Avenue
Orlando, FL 32803
13 of 23
7751 Kingspointe Parkway
Orlando, FL 32819
14 of 23
2555 S Kirkman Road
Orlando, FL 32811
15 of 23
2323 S Orange Avenue
Orlando, FL 32806
16 of 23
8972 Turkey Lake Road
Orlando, FL 32819
17 of 23
4780 N Orange Blossom Trl
Orlando, FL 32810
18 of 23
8119 S Orange Avenue
Orlando, FL 32809
19 of 23
12421 South Orange Blossom Trail
Orlando, FL 32837
20 of 23
3101 W Princeton Street
Orlando, FL 32808
21 of 23
7300 Sandlake Commons Blvd 205
Orlando, FL 32819
22 of 23
8865 Commodity Cir 13-216
Orlando, FL 32819
23 of 23
2415 W Sand Lake Road
Orlando, FL 32809
keyboard_arrow_left
keyboard_arrow_right

Drug Testing in Orlando, Florida

Orlando FL Drug Testing Locations

Get Drug Testing in Orlando, Florida Right Now

Health Street has the largest selection of drug testing and employment screening services in Orlando. Employers can access our services to set up a drug free workplace, families can get court ordered drug testing, and attorneys and private individuals can register for non-DOT drug tests, Department of Transportation (DOT) drug tests, and more.

Available Drug Testing Methods

content_cut
Learn about our Hair Follicle Drug Tests
gradient
See a full list of our Urine Drug Testing services.

Popular Drug Testing Panels

Specialized Categories of Tests

arrow_right
For companies that don’t wish to include marijuana, we have a slate of drug tests without marijuana
arrow_right
Choose from our Alcohol Testing options (such as breathalyzer, EtG test, or PEth blood alcohol)
arrow_right
Read more about our Court Ordered Drug Testing for individuals and families.

DOT Drug Testing

Learn about our DOT drug and alcohol testing services, and how your company can maintain Department of Transportation (DOT) Compliance with 46 CFR Part 40, including:

directions_transit
directions_transit
directions_transit
directions_ferry

Employee Drug Testing: eResults Portal for Employers

Business users get access to the amazing StaffGlass™ portal. When logged in, human resources and other company executives can manage their drug testing program, including:

arrow_right
Register for drug screenings for reasons such as: pre-employment, random, post-accident, or reasonable suspicion.
arrow_right
Get fast, accurate results in a safe and secure online portal.
arrow_right
Find a drug test clinic in Orlando, FL or pick from our lab testing locations anywhere in the United States; you can order your test online, view results, and check status of all tests
arrow_right
Set up State Background Check and employment background screening
arrow_right
Register staff for Occupational Health Services
arrow_right
Manage eSign documents related to the testing process
arrow_right
Create an employee pool for random drug testing, add or remove staff, update staff employment status.
arrow_right
StaffGlass™ is backed up by world class customer service.
arrow_right
Call Center Hours: Monday through Friday: 7:30 AM to 7:00 PM Eastern Time.

Why Health Street

A Decade Plus of Experience

Health Street has provided drug tests since 2010. The first time we performed drug testing in Orlando, FL was in 2013.  From then until now, we have completed 648 drug tests at our Orlando labs. 

Dedicated to Excellence

We are dedicated to providing a level of excellence in drug testing in Orlando that is unmatched. 

Substance Abuse Trends in Orlando

Central Florida Community Benefit Collaborative conducted a 2019 survey of the local population with a history of drug and alcohol abuse. The survey report indicated that there are problems that prohibit people from getting the treatment they need.

The areas of concern identified by the report included (a) a lack of state funding for treatment facilities for those experiencing abuse disorders; (b) a need for more education on the dangers of vaping and tobacco use; (c) issues with getting into treatment facilities, and; (d) proper accommodations specific to treatment for youth. 

Healthcare Has Stolen Disney’s Crown as Industry Leader in Orlando

In 2019, the Bureau of Labor Statistics ranked Orlando first in the nation for job growth for the fourth year in a row. In recent years, the City of Orlando (and Orange County, on a broader level) received accolades and recognition for economic development, particularly around job creation and business-friendliness.

Despite its reputation as a Disney town, Universal Orlando is the second biggest employer in the city with 25,000 employees. The largest employer is AdventHealth with nearly 29,000 employees; Orlando Health employs 19,000. Like many cities across the country, “The City Beautiful” is anticipating a healthcare-dominant economy over the next ten years. By 2030, the Orlando Economic Partnership expects home and healthcare services to see a 64% increase in employment in the area.

Local Diversion Programs

In 2019, the City of Orlando passed a cannabis diversion program to reduce arrests and get substance abuse victims into treatment. The program is aimed at low-level civil offenses like possession of marijuana, which has been largely decriminalized in Orlando and legalized for medical use in the State of Florida.

First and second-time offenders can choose to complete eight hours of community service or an eight hour substance abuse training course instead of paying a fine or going to court. Criminal charges are dropped upon successful completion of the program.

Locations

Clinic Hours and Information for our Labs in Orlando, Florida

2200 N Alafaya TrlSuite 900Orlando, FL 32826
Pre-registration required,Walk-in with barcode:

Monday: 8:00 AM – 2:00 PMTuesday: 8:00 AM – 2:00 PMWednesday: 8:00 AM – 2:00 PMThursday: 8:00 AM – 2:00 PMFriday: 8:00 AM – 2:00 PM About this Location: Our clinic is just a few blocks from Lake Rouse.
4401 S Orange AvenueSuite 110Orlando, FL 32806
Pre-registration required,Walk-in with barcode:

Monday: 6:30 AM – 3:00 PMTuesday: 6:30 AM – 3:00 PMWednesday: 6:30 AM – 3:00 PMThursday: 6:30 AM – 3:00 PMFriday: 6:30 AM – 3:00 PM About this Location: It is simple to locate our lab. We are a short walk from Cypress Grove Park.
5425 S Semoran BlvdSuite 7aOrlando, FL 32822
Pre-registration required,Walk-in with barcode:

Monday: 8:00 AM – 2:30 PMTuesday: 8:00 AM – 2:30 PMWednesday: 8:00 AM – 2:30 PMThursday: 8:00 AM – 2:30 PMFriday: 8:00 AM – 2:30 PM About this Location: You can locate our clinic down the block from Mc Coy Elementary School.
7300 Sandlake Commons BlvdSuite 205Orlando, FL 32819
Pre-registration required,Walk-in with barcode:

Monday: 8:00 AM – 2:30 PMTuesday: 8:00 AM – 2:30 PMWednesday: 8:00 AM – 2:30 PMThursday: 8:00 AM – 2:30 PMFriday: 8:00 AM – 2:30 PM About this Location: Our lab is located around the corner from Orange County Convention Center.
2504 S Alafaya TrlSuite 160Orlando, FL 32828
Pre-registration required,Walk-in with barcode:

Monday: 7:30 AM – 3:00 PMTuesday: 7:30 AM – 3:00 PMWednesday: 7:30 AM – 3:00 PMThursday: 7:30 AM – 3:00 PMFriday: 7:30 AM – 3:00 PM About this Location: It is simple to locate our lab. We are very close by to Anytime Fitness.
1603 S Hiawassee RoadSuite 120Orlando, FL 32835
Pre-registration required,Walk-in with barcode:

Monday: 6:30 AM – 3:30 PMTuesday: 6:30 AM – 3:30 PMWednesday: 6:30 AM – 3:30 PMThursday: 6:30 AM – 3:30 PMFriday: 6:30 AM – 3:30 PM About this Location: This office is conveniently located a short walk from MetroWest Golf Club.
14050 Town Loop BlvdSuite 105Orlando, FL 32837
Pre-registration required,Walk-in with barcode:

Monday: 6:00 AM – 3:00 PMTuesday: 6:00 AM – 3:00 PMWednesday: 6:00 AM – 3:00 PMThursday: 6:00 AM – 3:00 PMFriday: 6:00 AM – 3:00 PMSaturday: 7:00 AM – 11:00 AM About this Location: You can find us close to Suki Hanna.
114 Ernestine StreetOrlando, FL 32801
Pre-registration required,Walk-in with barcode:

Monday: 8:30 AM – 4:00 PMTuesday: 8:30 AM – 4:00 PMWednesday: 8:30 AM – 4:00 PMThursday: 8:30 AM – 4:00 PMFriday: 8:30 AM – 4:00 PM About this Location: You can locate our clinic very close by to Lake Lucerne.
4632 36th StreetSuite 405Orlando, FL 32811
Pre-registration required,Walk-in with barcode:

Monday: 9:00 AM – 3:30 PM(M Lunch: 12:30-1:30 PM)Tuesday: 9:00 AM – 3:30 PM(T Lunch: 12:30-1:30 PM)Wednesday: 9:00 AM – 3:30 PM(W Lunch: 12:30-1:30 PM)Thursday: 9:00 AM – 3:30 PM(Th Lunch: 12:30-1:30 PM)Friday: 9:00 AM – 3:30 PM(F Lunch: 12:30-1:30 PM) About this Location: You can locate our clinic just a few blocks from Dead Lizard Brewing Company.
7780 Lake Underhill RoadOrlando, FL 32822
Pre-registration required,Walk-in with barcode:

Monday: 7:00 AM – 2:30 PMTuesday: 7:00 AM – 2:30 PMWednesday: 7:00 AM – 2:30 PMThursday: 7:00 AM – 2:30 PMFriday: 7:00 AM – 2:30 PM About this Location: For urine drug testing in Orlando, Florida, you can find us a short walk from AdventHealth East Orlando ER.
1430 N Mills AvenueUnit 140Orlando, FL 32803
Pre-registration required,Walk-in with barcode:

Monday: 6:30 AM – 3:30 PMTuesday: 6:30 AM – 3:30 PMWednesday: 6:30 AM – 3:30 PMThursday: 6:30 AM – 3:30 PMFriday: 6:30 AM – 3:30 PMSaturday: 7:00 AM – 11:00 AM About this Location: Visit us at this location. We are nearby to Ten10 Brewing Company.
626 N Thornton AvenueOrlando, FL 32803
Pre-registration required,Walk-in with barcode:

Monday: 7:00 AM – 4:00 PMTuesday: 9:00 AM – 6:00 PMWednesday: call for hoursThursday: 9:00 AM – 6:00 PMFriday: 7:00 AM – 2:00 PM About this Location: Our lab is located across from Russian Ballet Orlando. You can’t miss us.
7751 Kingspointe ParkwayOrlando, FL 32819
Pre-registration required,Walk-in with barcode:

Monday: 8:00 AM – 5:00 PMTuesday: 8:00 AM – 5:00 PMWednesday: 8:00 AM – 5:00 PMThursday: 8:00 AM – 5:00 PMFriday: 8:00 AM – 5:00 PM
2555 S Kirkman RoadOrlando, FL 32811
Pre-registration required,Walk-in with barcode:

Monday: 8:00 AM – 5:00 PMTuesday: 8:00 AM – 5:00 PMWednesday: 8:00 AM – 5:00 PMThursday: 8:00 AM – 5:00 PMFriday: 8:00 AM – 5:00 PMSaturday: 8:00 AM – 4:00 PM
2323 S Orange AvenueOrlando, FL 32806
Pre-registration required,Walk-in with barcode:

Monday: 8:00 AM – 5:00 PMTuesday: 8:00 AM – 5:00 PMWednesday: 8:00 AM – 5:00 PMThursday: 8:00 AM – 5:00 PMFriday: 8:00 AM – 5:00 PMSaturday: 8:00 AM – 4:00 PMSunday: 8:00 AM – 4:00 PM
8972 Turkey Lake RoadOrlando, FL 32819
Pre-registration required,Walk-in with barcode:

Monday: 8:00 AM – 8:00 PMTuesday: 8:00 AM – 8:00 PMWednesday: 8:00 AM – 8:00 PMThursday: 8:00 AM – 8:00 PMFriday: 8:00 AM – 8:00 PMSaturday: 8:00 AM – 8:00 PMSunday: 8:00 AM – 8:00 PM About this Location: We are close to Phillips Village. We are easily accessed by car or public transportation.
4780 N Orange Blossom TrlOrlando, FL 32810
Pre-registration required,Walk-in with barcode:

Monday: 7:30 AM – 5:00 PMTuesday: 7:30 AM – 5:00 PMWednesday: 7:30 AM – 5:00 PMThursday: 7:30 AM – 5:00 PMFriday: 7:30 AM – 5:00 PM About this Location: We are a short walk from Lake Fairview Park. We look forward to serving you here.
8119 S Orange AvenueSuite 132Orlando, FL 32809
Pre-registration required,Walk-in with barcode:

Monday: 8:00 AM – 5:00 PMTuesday: 8:00 AM – 5:00 PMWednesday: 8:00 AM – 5:00 PMThursday: 8:00 AM – 5:00 PMFriday: 8:00 AM – 5:00 PM About this Location: You can locate our clinic down the block from Southport Community Park.
12421 South Orange Blossom TrailOrlando, FL 32837
Pre-registration required,Walk-in with barcode:

Monday: 7:00 AM – 5:00 PMTuesday: 7:00 AM – 5:00 PMWednesday: 7:00 AM – 5:00 PMThursday: 7:00 AM – 5:00 PMFriday: 7:00 AM – 5:00 PM About this Location: You can find us very close by to Taste of Peru.
3101 W Princeton StreetOrlando, FL 32808
Pre-registration required,Walk-in with barcode:

Monday: 7:00 AM – 3:30 PMTuesday: 7:00 AM – 3:30 PMWednesday: 7:00 AM – 3:30 PMThursday: 7:00 AM – 3:30 PMFriday: 7:00 AM – 3:30 PMSaturday: 7:00 AM – 2:00 PM About this Location: Our lab is located a short walk from Orlando Science Schools – Middle School Campus.
7300 Sandlake Commons Blvd 205Orlando, FL 32819
Pre-registration required,Walk-in with barcode:

Monday: 6:30 AM – 3:00 PMTuesday: 6:30 AM – 3:00 PMWednesday: 6:30 AM – 3:00 PMThursday: 6:30 AM – 3:00 PMFriday: 6:30 AM – 3:00 PM About this Location: We are located just a few blocks from MedPlex Dental. There is plenty of parking available.
8865 Commodity Cir 13-216Orlando, FL 32819
Pre-registration required,Walk-in with barcode:

Monday: 8:30 AM – 4:30 PM(M Lunch: 12:30-2 PM)Tuesday: 8:30 AM – 4:30 PM(T Lunch: 12:30-2 PM)Wednesday: 8:30 AM – 4:30 PM(W Lunch: 12:30-2 PM)Thursday: 8:30 AM – 4:30 PM(Th Lunch: 12:30-2 PM)Friday: 8:30 AM – 4:30 PM(F Lunch: 12:30-2 PM)
2415 W Sand Lake RoadOrlando, FL 32809
Pre-registration required,Walk-in with barcode:

Monday: 9:00 AM – 4:00 PMTuesday: 9:00 AM – 4:00 PMWednesday: 9:00 AM – 4:00 PMThursday: 9:00 AM – 4:00 PMFriday: 9:00 AM – 4:00 PM

Some Orlando Neighborhoods That We Serve

location_city
Azalea Park
location_city
College Park
location_city
Central Business District
location_city
Holden Heights
location_city
Delaney Park
location_city
Lake Eola Heights
location_city
MetroWest
location_city
Thornton Park
location_city
Uptown
location_city
Williamsburg

Additional Services

Health Street offers a variety of other services, including:

playlist_add
Florida Background Check for businesses hiring in Orlando, FL

Local Reviews

Star Rating
By Max Nevin on 3/6/2018
Fast, helpful and detailed. I recieved many detailed texts throughout the 5 panel drug test i ordered and it was a life saver.
Star Rating
By Charla on 2/20/2018
American Mulch and Groundcover LLC
Prompt reliable service as always. Works well, no downtime. Personalized prompt service
Star Rating
By Rob on 1/4/2018
Great communication, very professional
Star Rating
By Daniel Bonilla on 12/1/2017
InProduction
Great customer service. Thanks for the follow up on the Puerto Rico review.
Star Rating
By Camille Norman on 8/8/2017
Great job! Delenia got exactly what I needed, was very pleasant and professional and got it to me extremely quick! Love it!
Star Rating
By Jason R Fragale on 5/19/2016
(A) customer service was AAAwesome!!! She is the WO-MAN!!!! Can’t say she is the man because that would be weird. (B) Orlando, Florida. (C) 5-Panel drug test. I would totally be her friend!!!
Star Rating
By Norbert falasco on 1/8/2016
excellent and patient.

Citations

menu_book
“Orlando Leads Nation in Job Growth Four Years in a Row.” Orlando News: Amplified, 25 January 2019, https://news.orlando.org/blog/orlando-leads-nation-in-job-growth-four-years-in-a-row/.
menu_book
“City of Orlando.” Orlando Economic Partnership, https://www.orlandoedc.com/Why-Orlando/About-the-Region/City-of-Orlando.aspx.
menu_book
“2030: Insight Into Orlando’s Future.” Orlando Economic Partnership, January 2019, https://info.orlandoedc.com/hubfs/2030report.pdf?hsCtaTracking=d3c38513-9e66-4b87-8a42-922d7eeac13a%7C3ea21d81-b590-4248-8317-85747fadd423.
menu_book
“Community Health Needs, 2019 Assessment.” Aspire Health Partners, 28 June 2019, https://aspirehealthpartners.com/wp-content/uploads/2019/07/Aspire-Health-Partners-2019-CHNA-FINAL-REPORT-6-28-19-REVISED-07-24-19.pdf.
menu_book
“Sign up for the Cannabis Diversionary Program.” City of Orlando, https://www.orlando.gov/Public-Safety/OPD/Sign-up-for-the-Cannabis-Diversionary-Program.