Drug Test - Louisville, KY

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Health Street provides drug and alcohol testing in Louisville, Kentucky at 26 drug testing centers. Whether you need drug and alcohol testing for your employees, yourself, or a family member, you can rest assured that Health Street drug testing services in Louisville, KY provide a safe and clean environment and accurate results. We'll send a barcode authorization for the urine or hair drug test that you choose by text and email, along with a map to the clinic address.

Register for urine or hair drug tests in Louisville, KY quickly and easily online. Drug testing panels include Department of Transportation (DOT) and non-DOT urine drug tests, breath alcohol tests, EtG alcohol tests, and hair drug testing. Test results are fast, reviewed by our Medical Review Officer (an MRO), and reported safely and securely online. You can also call (502) 205-7541 with any questions about our employment screening services.

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Workforce Screening, Digitally Delivered

“ Awesome Job ”

Awesome job!
ANTOINE M.
10/5/2026
EAGLE AXIS TRUCKING LLC

“ Buena aTensin ”

Muy buena aTensin
Rafael G.
9/30/2026
Spirit Logistics Network Inc

“ Great, Friendly ”

Great very friendly
William S.
9/29/2026
Walbert Trucking

“ Excelente Experiencia ”

Excelente la experiencia ya me realize los exmenes y todo me pareci de maravilla
Victor Andres H.
9/25/2026
Road Dog Industrial

“ Buena Atencin ”

Muy buena la atencin
Victor Andres H.
9/25/2026
Road Dog Industrial
Star Rating
4.9 out of 5 stars (66 reviews)

Find a Health Street Clinic

Drug Testing Services in Louisville, Kentucky

Louisville KY Drug Testing Locations

Types of Drug Tests at our Louisville, KY laboratories:

At our Louisville drug testing locations, Health Street offers the most types of drug testing services to meet anyone's needs. Employers, courts, attorneys, and private individuals can choose from a wide variety of non-DOT or DOT drug and alcohol tests via rapid drug test or lab testing with the ability to detect virtually any substance.

Available Testing Methods

DOT Drug Testing services:

What You Can Do in the GLASS Portal

Our GLASS portal brings together everything employers need to manage testing and screening. Inside your account, you’ll be able to:

  • Register drug and alcohol tests at thousands of clinic locations nationwide
  • Run and review background checks
  • Set up occupational health appointments for staff
  • Collect and manage signed testing documents digitally
  • Maintain DOT random pool memberships and stay audit-ready

Why Health Street

Over a Decade of Experience

Health Street has been performing drug testing nationally since 2010. The first Louisville, KY drug test was provided by Health Street in 2014. In fact, we have successfully completed 936 drug tests at our Louisville testing centers since then.

Dedication to Excellence

We are committed to providing excellent services and to helping businesses and families obtain the drug testing in Louisville that they require.

Drug Testing in Louisville, KY

Statistics in Louisville, KY

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Jefferson County, which includes Louisville, experienced the highest number of overdose deaths involving fentanyl in Kentucky in 2021, with 477 deaths reported.
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Fentanyl was identified in 72.8% of the total drug overdose deaths statewide, with a 16% increase from 2020 to 2021​​.
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Methamphetamine was present in approximately 48% of overdose deaths, indicating a 33% increase from the 809 OD deaths involving methamphetamine in 2020​​.
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Nonfatal Drug-involved overdose Emergency Department (ED) visits in Kentucky totaled 12,946 in 2021, a slight increase from 2020, while nonfatal opioid-involved overdose ED visits saw a 7.8% decrease​​.

Local Initiatives to Curb Substance Abuse

The Kentucky Office of Drug Control Policy (ODCP) has implemented several initiatives to combat substance abuse in Louisville and across the state. One notable program is the Recovery Ready Communities Certification Program, designed to improve substance use disorder (SUD) recovery efforts within communities. The Medication Oriented Recovery and Enhancement (MORE) Program is another initiative to help people struggling with opioid use disorder recover with medication-assisted treatments. Louisville also has harm reduction programs, safe syringe disposal bins, and resources to request fentanyl test strips.

Local Drug Busts or Events

In January 2024, there was a drug raid in Pleasure Ridge Park in Louisville, KY. Louisville Metro Police Department's 3rd Division officers conducted a bust in the 4600 block of Seibel Court, spurred by a disturbance call received earlier that day. Upon arrival, officers encountered a 23-year-old, who initially claimed to have been assaulted by a woman inside the home, a claim that investigators later disputed.

The situation escalated when officers found drugs, guns, and ammunition within the residence. Further investigation revealed involvement in trafficking various narcotics, with evidence including multiple types of drugs and firearms discovered in a charcoal grill outside and a safe inside the home. The confiscated items included suspected methamphetamine, cocaine, heroin, fentanyl, mushrooms, marijuana, and pills, all seemingly prepared for sale.

The man admitted to selling illegal narcotics as his primary source of income and using a Durango for transportation. He was charged with strangulation, assault, and numerous felony drug charges, leading to his arraignment and a $75,000 cash bond at Louisville Metro Corrections.

Locations in Louisville, KY

Hours of Operation for our Louisville, Kentucky Labs

Neighborhoods in Louisville Served by our Clinics

Downtown
Old Louisville
East Market Highlands
Portland
Fern Creek
Pleasure Ridge Park
Fairdale
Okolona
Valley Station
Butchertown
Park Duvalle

Related Employment Screening Services

Health Street offers a variety of other services, including:

Reviews

Professional Quickness

Star Rating
By Richard F. on 8/31/2026
Enovation Controls
They were professional and quick

Amazing, Knowledgeable, Friendly

Star Rating
By Jorge V. on 8/16/2026
Staff was Amazing, Knowledgable, and Friendly! Was attended quickly! The Nurse and Doctor were Cheerful, Friendly and explained everything I needed to know. I highly recommend them. Open 247 Monday - Sunday! Thank you !!!!!

Good People

Star Rating
By Arian R. on 8/10/2026
Spirit Logistics Network Inc
Good people

Very Nice

Star Rating
By Victor S. on 8/7/2026
Spirit Logistics Network Inc
Was very nice

Clean, Professional

Star Rating
By Marcus A. on 7/16/2026
Spirit Logistics Network Inc
Clean and professional

Good Service

Star Rating
By Miguel U. on 7/15/2026
SLB Freightage Inc
Good service

Good, Quick

Star Rating
By Ryan A. on 7/13/2026
It didnt take long. Good

Exelente Trabajo

Star Rating
By Yurien G. on 6/28/2026
Spirit Logistics Network Inc
Exelente trabajo

Buena Atencin

Star Rating
By Orlando H. on 6/17/2026
Spirit Logistics Network Inc
Muy buena atencin

Fast, Polite Service

Star Rating
By Peter F. on 5/20/2026
Skybox Packaging LLC
Fast, easy, in and out, polite people.